Table 1.

Major characteristics of strains CVO and FWKO B isolated from oil field brine

MorphologySlightly curved rodsSlightly curved rods
Width (μm)0.40.4
Length (μm)0.6–0.81.0–2.0
Gram stain
Blood agar
Oxidase test+
Aerobic growth
 Acetate, succinate, lactate, fumarate, malate, formate, pyruvate, glucose, glycerol
Microaerophilic growth (1% O2)
Anaerobic growth on NO3 by oxidation of:
 Acetate, pyruvate, succinate, fumarate, lactate, propionate, glucose
Anaerobic growth on NO223 by oxidation of:
Anaerobic growth on N2O (2%) by oxidation of HS+c
Anaerobic growth on H2 by reduction of S0+
Anaerobic growth on acetate by reduction of S0ND
Growth by fermentation of pyruvate, succinate, lactate, fumarate, malate, aspartate
  • a +, positive; −, negative.

  • b Nonmotile or only weakly motile.

  • c Only a fivefold increase in cell numbers was observed after 5 days of incubation.

  • d ND, not determined.