Table 1.

Major characteristics of strains CVO and FWKO B isolated from oil field brine

ParameterValuea
CVOFWKO B
MorphologySlightly curved rodsSlightly curved rods
Width (μm)0.40.4
Length (μm)0.6–0.81.0–2.0
Spores
Motilityb +
Gram stain
Blood agar
Oxidase test+
H2O2decomposition
Aerobic growth
 Acetate, succinate, lactate, fumarate, malate, formate, pyruvate, glucose, glycerol
 S2O3 2−
 S0 NDd
Microaerophilic growth (1% O2)
 HS ++
 S0 +ND
 H2 ND+
Anaerobic growth on NO3 by oxidation of:
 HS ++
 S2O3 2−
 S0 +
 H2 +
 Formate+
 Acetate, pyruvate, succinate, fumarate, lactate, propionate, glucose
Anaerobic growth on NO2 23 by oxidation of:
 HS +
 S0 +
 H2 ND
Anaerobic growth on N2O (2%) by oxidation of HS +c
Anaerobic growth on H2 by reduction of S0 +
Anaerobic growth on acetate by reduction of S0 ND
Growth by fermentation of pyruvate, succinate, lactate, fumarate, malate, aspartate
  • a +, positive; −, negative.

  • b Nonmotile or only weakly motile.

  • c Only a fivefold increase in cell numbers was observed after 5 days of incubation.

  • d ND, not determined.