Table 2.

Oligonucleotide probes applied

ProbeSpecificitySequence (5′ to 3′) of proberRNA target sitea% FAb[NaCl] (mM)c
NSO1225Ammonia-oxidizing β-Proteobacteria CGCCATTGTATTACGTGTGA 16S, 1225–12443580
NSV443 Nitrosospiraspp. CCGTGACCGTTTCGTTCCG 16S, 444–46230112
NSR826 Nitrospiraspp. GTAACCCGCCGACACTTA 16S, 826–84320225
NSR1156 Nitrospiraspp. CCCGTTCTCCTGGGCAGT 16S, 1156–117330112
NSR447 Nitrospiraspp. GGTTTCCCGTTCCATCTT 16S, 447–46430112
  • a Escherichia coli numbering (9).

  • b Formamide in the hybridization buffer.

  • c Sodium chloride in the washing buffer.