Table 1.

MPN counts of bacteria in anoxic soil of a flooded rice paddy soil microcosm

SubstrateCell count (per g of dry soil)95% Confidence intervalStrain isolated in pure culture
Lactatea 3.7 × 106 8.3 × 105–1.1 × 107 Desulfovibriosp. strain DR10
Ethanola 2.3 × 106 4.4 × 105–8.3 × 106 Desulfovibriosp. strain DR14
Succinate5.9 × 105 9.8 × 104–2.9 × 106 Desulfovibriosp. strain DR1
Betainea 5.9 × 104 9.8 × 103–2.9 × 105 Desulfobacteriumsp. strain DR7
Ethylene glycol1.0 × 104 2.0 × 103–4.4 × 104 Sporomusasp. strain DR6
2,3-Butanediol1.0 × 104 2.0 × 103–4.4 × 104 Sporomusasp. strain DR16
3,4,5-TMBa,b 2.3 × 103 4.4 × 102–8.3 × 103 Sporomusasp. strain DR5
  • a Three further counts with soil from different microcosms were made with these substrates (two incubated at 25°C and one incubated at 15°C); in all cases, the counts were not significantly different (P < 0.05) from those obtained in the experiments listed in the table.

  • b 3,4,5-TMB, 3,4,5-trimethoxybenzoate.