Table 2.

T-RFLP OTU fragment sizesa

IsolateMean OTU fragment size (bp) ± SD
Single templateMixture of all 6 templates
MS-01352 ± 3352 ± 3
MS-02213 ± 2208 ± 2
MS-20223 ± 2222 ± 2
MS-21115 ± 4118 ± 4
MS-22326 ± 1326 ± 1
MS-23582 ± 5583 ± 5
E. coli 359 ± 1 NIb
  • a Shown are sizes of T-RFLP OTU fragments from six marine strains digested with two restriction enzymes (CfoI and RsaI) and sizes of T-RFLP OTU fragments of each marine snow isolate (single template; n = 8) and T-RFLP OTUs when all six templates were present in one PCR mixture (combination of all six templates; n = 4).

  • b NI, not included in reaction mixture.