Table 1.

Descriptions of samples collecteda

SampleSampling date (day/mo/yr)Latitude/longitudeDepth (m)Sample collection depth (m)chl a (μg liter−1)No. of OTUs
CfoIMvnIRsaIDGGE
an112/8/9543°39.00/13°35.00200.50.75393532 NDb
an213/08/9543°39.00/13°35.00200.51.02423942ND
an314/08/9543°39.00/13°35.00200.50.85385145ND
an415/08/9543°39.00/13°35.00200.51414236ND
an506/9/9543°39.00/13°35.00200.50.79323128ND
an6011/9/9543°39.00/13°35.00200.51.55354939ND
an81624/09/9543°39.00/13°35.00200.51.35264443ND
msb18/3/9836°04.50/25°17.001,740100.1744423935
msb1rt8/3/9836°04.50/25°17.001,740100.17424438ND
msb37/3/9836°00.00/23°53.601,190100.1844423935
mnb114/03/9840°14.90/25°12.001,300100.6443383633
mnb1rt14/03/9840°14.90/25°12.001,300100.64514642ND
mnb215/03/9840°04.65/24°36.20960100.6344393833
mnb2rt15/03/9840°04.65/24°36.20960100.63584746ND
mnb313/03/9839°13.45/25°00.00820100.1740424036
mnb419/03/9839°47.10/25°31.5095100.3741393328
ro12/5/9545°05.00/13°33.00250.50.75463539ND
ro25/5/9545°05.00/13°33.00250.50.8463045ND
ro36/5/9545°05.00/13°33.00250.51363436ND
ro119/5/9545°05.00/13°33.00250.51.2473044ND
  • a Samples from the upper mixed water column were collected in the Middle Adriatic Sea off Ancona (an; Italy), in the South (msb) and North (mnb) Aegean Sea (Greece), and in the Northern Adriatic Sea off Rovinj (ro; Croatia). The number of OTUs found when the sample was digested with the respective tetrameric restriction enzyme (CfoI, MvnI, orRsaI) and in the DGGE gel is shown. For each sample, the patterns from the three tetrameric restriction digests were combined and used for UPGMA analysis (see Fig. 5).

  • b ND, not determined.