Table 1.

Adhesion and penetration of V. shiloi onto and into O. patagonicaa

Time after infection (h)% Adhesionb ± SE% Penetrationc ±SE
436 ± 100.9 ± 0.5
883 ± 05.6 ± 2.0
1277 ± 1620.9 ± 8.5
2486 ± 721.7 ± 7.6
48100 ± 05.3 ± 4.3
72100 ± 00.0 ± 0
  • a An overnight culture of V. shiloi was inoculated into 125-ml flasks containing healthy pieces of coral in 25 ml of sterile seawater. The flasks were incubated at 29°C with gentle shaking (80 rpm) with cycles alternating 12 h of light and 12 h of darkness. Data are averages from three experiments, each performed in duplicate with initial bacterial concentrations of ca. 4 × 106 per ml.

  • b Calculated from CFU lost from the seawater.

  • c Calculated from the CFU in the crushed coral (compared to the inoculated bacteria) following treatment of the coral with methyl-β-d-galactopyranoside and gentamicin.