Table 1.

Oligonucleotide probes used for FISH

ProbeSpecificityProbe sequence (5′–3′)Target sitea 16S rRNA positions% FAbSource or reference
ALT1413Alteromonas,ColwelliaTTTGCATCCCACTCCCAT1413–143040This study
G RbRhodobacter,RoseobacterGTCAGTATCGAGCCAGTGAG645–6263018
G VVibrioAGGCCACAACCTCCAAGTAG841–8223018
NOR1-56NOR1 lineageTTACCGCTCGGACTTGCA56–7320This study
NOR2-1453NOR2 cluster group AGGTCATCGCCATCCCC1453–146830This study
OCE232OceanospirillumAGCTAATCTCACGCAGGC232–24940This study
PSA184Pseudoalteromonas,ColwelliaCCCCTTTGGTCCGTAGAC184–21030This study
SAR86-1249SAR 86 clustercGGCTTAGCGTCCGTCTG1249–126550This study
SPH120SphingomonasGGGCAGATTCCCACGCGT120–1373036
  • a E. coli numbering (8).

  • b Percent (vol/vol) formamide (FA) in FISH buffer.

  • c Reference 35.