Table 2.

Experimental disassociation and stringency results for S-S-Thio-0820-a-A-22 and S-G-Acdp-0821-a-A-24

Probea and target groupNo. of mismatchesExperimental Td (°C) ± SDAvg nontarget signal intensity as % of target intensity (range)
S-S-Thio-0820-a-A-22
T. thiooxidans ATCC 19377046.0 ± 0.17
T. thiooxidans DSM 612046.5 ± 0.42
T. thiooxidans ATCC 19703Unknown45.9 ± 0.53
T. thiooxidans ATCC 8085Unknown43.9 ± 0.47
T. ferrooxidans ATCC 19859046.8 ± 0.12
T. ferrooxidans ATCC 23270045.6 ± 0.12
 All target species45.8 ± 0.590.71 (0.25–1.31)
S-G-Acdp-0821-a-A-24
A. angustum ATCC 35903058.6 ± 0.12
A. cryptum ATCC 33463059.0 ± 0.35
A. organovorum ATCC 43141060.7 ± 0.17
A. acidophilum ATCC 27807062.2 ± 0.421.47 (0.30–11.12)
 GenusAcidiphilium 060.1 ± 1.65
A. diazotrophicus ATCC 49037252.1 ± 0.06
  • a Small-subunit rRNA positions (Escherichia coli numbering) are shown.