Table 4.

Effects of 0.1 M AlCl3 or 0.1 M MgCl3 and 4 M urea on virus adsorption to microporous filters at pH 3.5a

FilterVirus% Adsorption in the presence of:
BufferBuffer containing 4 M ureaBuffer containing 4 M urea plus 0.1 M MgCl2Buffer containing 4 M urea plus 0.1 M AlCl3
Millipore HAMS2>99 Ab 98 A15 B13 B
φX17498 A95 A9 B7 B
Poliovirus 1>99 A>99 A12 B15 B
Mean99971212
FiltriteMS2>99 A95 B14 B11 B
φX17495 A92 B7 B8 B
Poliovirus 199 A99 A13 B15 B
Mean98951111
WhatmanMS23 A9 A11 A7 A
φX1746 A4 A3 A6 A
Poliovirus 19 A7 A8 A4 A
Mean6776
1MDSMS295 A95 A10 B8 B
φX17485 A69 A48 B30 C
Poliovirus 199 A99 A50 B10 C
Mean93883616
  • a We added approximately 105PFU of virus to 25-ml portions of buffer, buffer containing 4 M urea, buffer containing 4 M urea plus 0.1 M MgCl2, and buffer containing 4 M urea plus 0.1 M AlCl3, and each type of solution was passed through the different types of filters at a rate of 1.5 ml/s. The numbers of viruses in the influent and effluent were measured in order to determine the percentage of adsorption in each case.

  • b Values on the same line followed by the same letter are not significantly different at P = 0.05.