Table 2.

Effect of 5- and 20-h supernatants of transformants in Vero cell cytotoxicity assay

Incubation time (h)aVero cell survival (mean % live cells ± SEM)b with:
TransformedE. coli JM109cTransformed Salmonella serovar EnteritidiscVesiclesd
547.3 ± 2.846.0 ± 3.064.9 ± 3.2
2036.4 ± 4.134.6 ± 3.968.9 ± 2.6
  • a Growth time in LB broth following transformation.

  • b Percent live cells was calculated as explained in Materials and Methods.

  • c Controls for the transformants were Vero cells treated with the appropriate bacteria (supernatant) and incubated for the same time under the same conditions.

  • d The control for vesicles was Vero cells treated with LB broth.