Table 1.

PCR amplification of the chuA andyjaA genes and DNA fragment TSPE4.C2 in E. colistrains from various collections according to phylogenetic group

Strain collection or isolates (no. of strains) ReferenceGroup as determined by reference methods (no. of strains)aNo. (%) of strains positive for amplification of:
chuAyjaATSPE4.C2
ECOR (68) 10 A (25)018 (72)b 0
B1 (16)01 (6)c 15 (94)c
D (12)12 (100)02 (17)d
B2 (15)15 (100)15 (100)12 (80)e
Neonatal meningitis (86) 4 A (5)05 (100)0
B1 (3)01 (33)2 (66)
D (18)18 (100)02 (11)
B2 (60)60 (100)60 (100)59 (98)
Verotoxin-producing E. coli O157:H7 (10)This studyD (10)10 (100)00
Other clinical strains (64)This studyA (12)09 (75)0
B1 (4)004 (100)
D (11)11 (100)01 (9)
B2 (37)37 (100)37 (100)34 (92)
E. coliK-12 10 A (1)010
E. coli J96This studyB2 (1)110
All strains (230)A (43)033 (77)0
B1 (23)02 (9)21 (91)
D (51)51 (100)05 (10)
B2 (113)113 (100)113 (100)105 (93)
  • a Phylogenetic groups were determined by multilocus enzyme electrophoresis or ribotyping.

  • b The strains negative for amplification ofyjaA were ECOR 4, ECOR 15, ECOR 16, ECOR 17, ECOR 22, ECOR 23, and ECOR 24.

  • c The strain negative for amplification ofyjaA and positive for amplification of TSPE4.C2 was ECOR 70.

  • d The strains positive for amplification of TSPE4.C2 were ECOR 49 and ECOR 50.

  • e The strains negative for amplification of TSPE4.C2 were ECOR 53, ECOR 59, and ECOR 60.