Table 1.

Primers used in this studya

PrimerSequence (5′–3′)TargetAnnealing temp (°C)Reference
Bac32FAACGCTAGCTACAGGCTT Bacteroides-Prevotella 53 2
Bac708RCAATCGGAGTTCTTCGTG Bacteroides-Prevotella 2
CF128FCCAACYTTCCCGWTACTCCF123 cluster58This study
CF193FTATGAAAGCTCCGGCCCF151 cluster55This study
HF134FGCCGTCTACTCTTGGCCHF1061This study
HF183FATCATGAGTTCACATGTCCGHF8 cluster, HF7459This study
HF654RCCTGCCTCTACTGTACTCHF1061This study
  • a Bac, Bacteroides-Prevotella; HF, human-specific; CF, cow-specific. Numbers correspond to the numbers of the E. coli 16S rRNA gene. All forward primers except HF134F were paired with Bac708R. HF134F was paired with HF654R. Annealing temperatures were empirically determined for each primer pair as described in the text.