Table 2.

Number of Collinsella and Atopobiumcluster bacteria and bifidobacteria in human feces determined by FISH using the probes COR653, ATO291, and BIF164

Group (n)aCollinsellagroupAtopobiumclusterBifidobacteria
No. of cellsb% Fluorescentc<0.5% (n)dNo. of cells% Fluorescent<0.5% (n)No. of cells% Fluorescent<0.5% (n)
Baby, 12 days
 Breast fed (6) NDe 0.5 (0–3)5ND0.5 (0–3)5ND68 (41–91)0
 Formula fed (6)ND17 (0–39)2ND20 (0–39)2ND33 (0.2–46)1
1–10 years (12)7.7 × 109 2.5 (0.1–8)31.0 × 1010 3.3 (0.2–12)11.8 × 1010 5.6 (0.4–12)1
25–55 years (10)4.4 × 109 1.1 (0.2–3)18.6 × 109 2.6 (0.1–7)18.9 × 109 2.5 (1.2–6)0
75–95 years (11)4.1 × 109 4.3 (0.3–9)31.1 × 1010 11.5 (0.3–26)01.1 × 1010 8.5 (0.1–20)2
  • a Groups were divided by age.

  • b Number of cells per gram of feces (dry weight). The coefficient of variation (CV) due to the assay error of the FISH method was 0.12 for automated counting (11) and 0.18 for visual counting (6).

  • c Percentage (range) of fluorescent cells relative to the number of total cells. Total-cell numbers were determined by phase-contrast microscopy (babies) or by DAPI staining (other samples).

  • d Number of volunteers that produced samples with no cells or less than 0.5% fluorescent cells relative to the number of total cells that hybridized with the indicated probe.

  • e ND, not determined. Absolute numbers could not be determined, since an unknown amount of feces was suspended in the transport medium (8).