Table 1.

Strains and plasmids used

Strain or plasmidRelevant propertiesReference
Strains
E. coliMC1061araD139(ara, leu)7697 ΔlacX74 galU galK hsr strA3
S. typhimuriumTN1547leuBCD485 pepN90 pepA16 pepB11 supQ302proAB pepD) pepP1 pepQ1 pepT1 pepE12
L. lactis subsp.cremoris
  MG1363Derivative of NCDO712, plasmid-free, prophage cured10
  IM16MG1363, pepX pepT pepO pepC pepN16
  UKLc10IM16,pepN::nisRKThis study
Plasmids
 pLP712lac+prt+, 33 MDa10
 pNZ8037Cmr, PnisA, pSH71 replicon5
 pNZ9573CmrErr, pepN::nisRK, p15a replicon4
 pJUK23Apr,pepL, ColE1 replicon12
 pJKG8Kmr, pepG, pSC105 replicon13
 pJK505Apr,pepI, ColE1 replicon14
 pJKW2Kmr, pepW, pSC105 replicon13
 pMS1Kmr,pepQ, pSC105 replicon22
 pKS3Kmr, brnQ, pSC105 replicon21
 pUK200pNZ8037 with terminator of brnQThis study
 pUK200QpUK200,PnisA::pepQThis study
 pUK200IpUK200,PnisA::pepIThis study
 pUK200LpUK200,PnisA::pepLThis study
 pUK200GpUK200,PnisA::pepGThis study
 pUK200WpUK200,PnisA::pepWThis study