Table 2.

Survival of L. lactis JIM4886 in the different compartments of the DTa

DT compartmentSurvival counts for bacteria that were:
Force-fedMixed with diet
109 CFU/ml1012CFU/ml
No. of bacteriabNo. of sporesb% SurvivalcNo. of bacteriaNo. of spores% SurvivalNo. of bacteriaNo. of spores% Survival
None (inoculum)1.0 × 109 4.8 × 108 1.0 × 109 4.8 × 108 1.0 × 1012 4.8 × 108
Stomach6.0 × 106 4.0 × 107 66.4 × 108 2.8 × 108 912.7 × 1011 1.2 × 108 90
Duodenum7.9 × 103 2.5 × 107 0.014.7 × 107 6.3 × 107 303.2 × 107 1.2 × 107 0.1
Jejunum4.0 × 105 4.0 × 107 0.42.8 × 108 2.6 × 108 434 × 108 1.0 × 108 0.2
Ileum4 × 107 4.0 × 107 404.5 × 108 2.6 × 108 644 × 109 1.6 × 108 1
Cecum7.9 × 106 2.0 × 107 165.0 × 108 2.0 × 108 1003.2 × 1011 2.0 × 108 64
  • a Bacteria were administered in suspension by force-feeding or mixed with the diet at 109CFU/ml or at 1012 CFU/ml. Spores of B. stearothermophilus were mixed with L. lactis cells at 4 × 108 spores/ml.

  • b Viable count expressed in CFU per milliliter of inoculum or CFU per milliliter of DT content.

  • c The survival percentage of L. lactis was calculated with respect to the spore counts: [(count of L. lactis in one DT compartment/count of spores in the same compartment)/(count of L. lactis in the inoculum/count of spores in the inoculum)] × 100.