Table 1.

Strains of E. coli used in the inoculum cocktail

StrainPathotypeSerogroupVirulence determinant(s)aSourceReference(s)Selective mediumb/ carbohydrate reaction
86-24STECO157:H7eae, Stx2Human15, 23SN dulcitol/positive
3081STECO157:H7eae, Stx1, Stx2Bovine11, 13KA sorbitol/negative
2041ETECO157:H43F4, F41, LT, STbPorcine52TN sorbose/positive
637ETECO64:NMF5, STaPorcine43, 54TN sorbose/negative
E2348/69EPECO127:H6eaeHuman39, 44SN dulcitol/negative
  • a LT, heat labile enterotoxin; STb, heat-stable enterotoxin b; STa, heat-stable enterotoxin a;eae, gene encoding intimin; F4, F4 (K88) fimbriae; F5, F5 (K99) fimbriae; F41, F41 fimbriae.

  • b MacConkey agar base; S, streptomycin (100 μg/ml); N, nalidixic acid (10 or 20 μg/ml); T, tetracycline (10 μg/ml); K, kanamycin (30 μg/ml); A, ampicillin (100 μg/ml).