Table 2.

Comparison of the amount of amplified DNA and the fluorescence signal in end point TNAsa with probes S8807 and S8807A

Expt and templatebAmplified DNAProbedFluorescence signal
Arbitrary areac%Avg arbitrary ΔRQ ± SD%
A
 BO 88077.192.2S88071.02 ± 0.17100
 BO 88087.394.8S88070.22 ± 0.0621.6
 BO 88057.192.2S88070.02 ± 0.042.0
 BO 94047.394.8S88070.17 ± 0.0416.7
A. variabilis  NDe NDS88070.03 ± 0.022.9
 BO 84024.659.7S88070.01 ± 0.031.0
Microcystissp.7.7100S88070 ± 0.040
E. coli 00S88070 ± 0.080
Anabaena strain PCC 7120NDNDS88070.01 ± 0.031.0
 None00S88070
B
 BO 88076.288.6S8807A0.8 ± 0.02100
 BO 88085.984.3S8807A−0.01 ± 0.04
 BO 88055.477.1S8807A−0.03 ± 0.04
 BO 94047.0100S8807A0.02 ± 0.032.5
  • a See Fig. 2.

  • b Template DNA (10 ng in experiment A, 1 ng in experiment B) was obtained from Synechococcus sp. strain BO 8805, BO 8807, BO 8808, and BO 9404; Synechococcus sp. strain BO 8402; two Anabaena strains; Microcystissp.; and E. coli.

  • c Area of ethidium bromide-stained PCR product (see Materials and Methods).

  • d Probe concentrations, 20 (S8807) and 50 (S8807A) nM.

  • e ND, three PCR products, areas not determined.