Table 2.

Southern blotting of representative Salmonellaserotype Typhimurium isolates and probe hybridization using selected gene cassettesa

Phage typeR typePresence (+) or absence (−) of 10-kb XbaI fragmentIP typeProbe hybridization
ant(3")-1apseIdhfrI
DT104
 CIT-V38ACSSuT+I++
 CIT-F45ACSSuT+I++
 CIT-H164ACSSuT+I++
 CIT-H176ACSSuTTp+I++
 CIT-H183SSuVII
 CIT-V115ACSSuT+I++
 CIT-F44ASuIII
DT104 related
 CIT-F107 (104b)ACSSuTK+I++
 CIT-H144 (104b)ACSSuTTp+I+++
 CIT-V37 (U302)ACSSuTTp+I++
DT193
 CIT-F34ASSuTNIV+++
 CIT-F41ASSuTTpII+++
DT195, CIT-V60SuTTpIV
DT208, CIT-V75TIV
DT170a
 CIT-V127ASSuTTpII+++
 CIT-V129SuTTpIV
NT
 CIT-F40SensitiveV
 CIT-F105SuTpV
 CIT-H195SuTpIV++
Controls
E. coli R100.1a+++
E. coli R751b++
  • a ant(3")-1a, aminoglycoside-modifying enzyme coding gene; pseI, β-lactamase gene; dhfrI, dihydrofolate reductase gene. R type, antimicrobial resistance pattern. IP types a and b were previously defined (6). A, ampicillin; C, chloramphenicol; S, streptomycin; Su, sulfonamides; T, tetracycline; N, nalidixic acid; K, kanamycin. In the phage types, the letters V, H, and F refer to veterinary, human, and food origins, respectively.