Table 1.

Bacterial strains and plasmids

Strain or plasmidRelevant characteristicsaSource or reference
Strains
E. coliHB2151Δlac-pro ara Nalrthi F′ (proAB lacIqlacZΔM15)9
E. coli XL1-BlueendA1 hsdR17 supE44 thi-1 recA1 gyrA96 relA1 lac F′(proAB lacIqlacZΔM15 Tn10)8
Plasmids
 pCANTAB-5EApr; vector for periplasmic production of scFv's with E-tag epitope at C terminusPharmacia
 pCANTAB-5EhisApr; pCANTAB-5E with His6 tag incorporated before E-tagThis work
 p6AC3g3Apr; scFv 6AC3 cloned betweenSfiI-NotI sites of pCANTAB-5EhisThis work
 pB4g3Apr; scFv B4 cloned betweenSfiI-NotI sites of pCANTAB-5EhisThis work
 pVDL9.3Cmr; production of HlyB and HlyD transporters35
 pLG612-1BApr; 23-kDa C-terminal domain of HlyA35
 pLG612-1SDApr; 23-kDa C-terminal domain of HlyA with Shine-Dalgarno sequence andNcoI site35
 pEHlyAApr; same as pLG612-1B with E-tag epitope incorporated at the 23-kDa C domain of HlyAThis work
 pEHlyA1SDApr; same as pLG612-1SD with E-tag epitope incorporated at the 23-kDa C domain of HlyAThis work
 pEHlyA2-SDApr; same as pEHlyA1SD with polylinker for cloning of scFv's in frame with E-tagged ′hlyAThis work
 p6AC3HLYAApr; scFv 6AC3 cloned betweenNcoI and XmaI sites in pEHlyA2-SDThis work
  • a Apr, ampicillin resistant; Cmr, chloramphenicol resistant.