Table 1.

Oligonucleotide PCR primers used in this study

PrimerSequenceaTargetbE. coli positionReference
Ar20-FTTC CGG TTG ATC CYG CCR GArchaeal SSU6–2534
GILSUT7-RGCC AGT GAA TTG TAA TAC GAC TCA CTA TAG GGC GCT TCC ATC AGG CAG AGGroup I LSU2610–2623This study
LSU190-FGAA YTG AAR CAT CTY AGT AProkaryote LSU190–207This study
LSU2445a-RCCC YGG GGT ARC TTT TCT STArchaeal LSU2432–2447This study
Eub27fAGA GTT TGA TCC TGG CTC AGBacterial SSU8–2718
LSUT71933e-RGCC AGT GAA TTG TAA TAC GAC TCA CTA TAG GGA CCC GAC AAG GAA TTT CGCBacterial LSU1933–1951This study
Ar20T7-FGCC AGT GAA TTG TAA TAC GAC TCA CTA TAG GGT TCC GGT TGA TCC YGC CRGArchaeal SSU6–25This study
Eub27T7-FGCC AGT GAA TTG TAA TAC GAC TCA CTA TAG GGA GAG TTT GAT CCT GGC TCA GBacterial SSU8–27This study
G1LSU-RCGC TTC CAT CAG GCA GAGGroup I LSU2610–2623This study
LSU1933e-RACC CGA CAA GGA ATT TCG CBacterial LSU1933–19514
  • a The T7 RNA polymerase promoter sequence is in boldface type.

  • b SSU, small subunit; LSU, large subunit.