Table 3.

Amount of DNA extracted from different soils after lysis treatments according to protocols 1 to 5a

Soil no. (origin)bAmt of DNA (μg/g [dry wt] of soil) ± SD extracted after lysis protocol:c
1234a4b5a5b
1. (Australia)17 ± 252 ± 232 ± 516 ± 333 ± 259 ± 127 ± 0
2. (Peyrat)29 ± 258 ± 140 ± 229 ± 218 ± 356 ± 115 ± 1
3. (Côte St André)36 ± 760 ± 6148 ± 1094 ± 738 ± 673 ± 547 ± 6
4. (Chazay)916NDd 32151570
6. (Dombes)4 ± 226 ± 343 ± 161 ± 166 ± 1160 ± 7102 ± 5
  • a Quantification was performed by phosphorimaging after dot blot hybridization with the universal probe FGPS431 (Table 2).

  • b For soils 1, 2, 3, and 6, n = 3 ; for soil 4, n = 1 .

  • c Protocols: 1, no treatment; 2, dry soil grinding; 3, dry soil grinding plus Ultraturrax homogenization; 4a, dry soil grinding, homogenization, and microtip sonication; 4b, dry soil grinding, homogenization, and Cup Horn sonication; 5a, dry soil grinding, homogenization, microtip sonication, and chemical and enzymatic lysis; 5b, dry soil grinding, homogenization, Cup Horn sonication, and chemical and enzymatic lysis. See also Fig. 1.

  • d ND, not determined.