Table 2.

Primary and secondary transcript half-lives for tested plasmidsa

PlasmidHalf-life (min)
Primary transcriptSecondary transcript (lacZ)Secondary transcript (gfp)
p50gl2.0 (±0.1)
p50gΔl8.0 (±0.3)
p50HP4gΔl7.6 (±0.3)
p50HP17gΔl7.5 (±0.5)
p60gHP4l3.5 (±0.2)3.5 (±0.2) [2.9 (±0.2)]b 2.9 (±0.1) [2.9 (±0.1)]
p70gHP4l4.0 (±0.2)6.9 (±0.4) [5.4 (±0.4)]3.9 (±0.4) [3.1 (±0.4)]
  • a Values for half-lives are given as mean values and standard errors obtained by method 1.

  • b Values in brackets are mean values and standard errors obtained by method 2.