Table 1.

Strains and plasmids used

Strain or plasmidPhenotype/descriptionReference or source
DH10BF mcrA Δ(mrr-hsdRMS-mcrBC) φ80dlacZΔM15 ΔlacX74 deoR recA1 endA1 araD139 Δ(ara, leu)7697 galU galK λ rpsL nupG Gibco BRL
JS4F araD139 Δ(ara,leu)7697 Δ(lac)χ74 galU galK hsdR2 (rk mk ) mcrA mcrBC rpsL(Strr) thi recA1 Bio-Rad
pGFPuvpUC-based plasmid (pUC19 ori);gfpuv gene under lac promoter control; Ampr Clontech
pGFPuvmpGFPuv with silent mutation in coding region to remove SalI restriction site and improve translation efficiencyThis study
pGFPuvm2pGFPuvm with silent mutation in coding region to remove XhoI restriction site and improve translation efficiencyThis study
pTC40pBAD24-based plasmid (pBR322 ori);lacZ under araC/PBAD promoter control; Ampr 6
pTC40mpTC40 with mutation in pretranslation region to replace Asp718 with NheI restriction siteThis study
p50glpTC40 with coding region from pGFPuvm2 and pretranslation region from pTC40m inserted 5′ of lacZ untranslated regionThis study
p50gΔlp50gl with lacZ coding region removedThis study
p50HP4gΔlp50gΔl with HP4 inserted 5′ of gfp This study
p50HP17gΔlp50gΔl with HP17 inserted 5′ of gfp This study
p60glp50gl with HP1 inserted 3′ of gfp and RNase E site (E1) inserted 5′ oflacZ This study
p60gHP4lp60gl with HP4 inserted 5′ of lacZ This study
p70glp50gl with HP1 inserted 3′ of gfp and RNase E site (E2) inserted 5′ oflacZ This study
p70gHP4lp70gl with HP4 inserted 5′ of lacZ This study
pGEM-4zCommercial transcription vector (SP6/T7 promoters); multicloning site withinlacZ′; Ampr Promega
pTC01pGEM-4z with 5′ section of lacZ coding region from pTC40 inserted into multicloning siteThis study
pCS01pGEM-4z withgfp coding region from pGFPuvm2 inserted into multicloning siteThis study
pCS02pGEM-4z with coding region from p60gHP4l inserted into multicloning siteThis study