Table 1.

Composition of the CDM

ConstituentbFinal concn (mM)a
MPLRcMPL (amt/liter)
Lactose58.458.4 (20 g)
Sodium thioglycolate* 4.384.38 (0.5 g)
Tween 80* (% vol/vol)0.10.1 (1 g)
K2HPO4 · 3H2O* 7
Na2HPO4 2.11 (0.3 g)
NaH2PO4 · H2O5.8 (0.8 g)
Potassium acetate* 1575 (7.35 g)
Ammonium citrate* 10
Orotic acid* 3.2
Pyruvic acid* 0.9
Formic acid* 0.2
Oleic acid* 0.033
NaCl10
CaCl2 * 52.5 (0.27 g)
MgSO4 · 7H2O* 0.8d 0.8 (0.2 g)
MnSO4 · H2O0.12e 0.12 (0.02 g)
FeSO4 · 7H2O (μM)70f 0.23 (0.066 mg)
Micronutrients* (μM)
 (NH4)6Mo7O24 · 4H2O0.003g 0.003 (0.0037 mg)
 CoCl2 · 6H2O0.03g 0.03 (0.007 mg)
 H3Bo3 0.4g 0.4 (0.025 mg)
 CuSO4 0.01g 0.01 (0.0016 mg)
 ZnSO4 · 7H2O0.01g 0.01 (0.0029 mg)
Bases*
 Adenine0.37e 0.37 (0.05 g)
 Guanine0.33e 0.33 (0.05 g)
 Xanthine0.320.32 (0.05 g)
 Uracil0.45e 0.45 (0.05 g)
 2′-Deoxyguanosine* 0.19e
 Hypoxanthine* 0.37
Amino acids
l-Alanine2.24d 1.12i (0.1 g)
l-Arginine0.29d 1.84i (0.32 g)
l-Asparagine1.9d 2.27 (0.3 g)
l-Aspartate7.53.75i (0.5 g)
l-Cysteine · HCl2.28d 1.6 (0.28 g)
 Glycine2.66d 2.13i (0.16 g)
l-Glutamate5.9d 2.25i (0.38 g)
l-Glutamine2d 2 (0.3 g)
l-Histidine3.22d 1.3i (0.2 g)
l-Isoleucine3.8d 2.75i (0.36 g)
l-Leucine1.52d 4.57i (0.6 g)
l-Lysine · HCl0.27d 2.4i (0.44 g)
l-Methionine3.35d 0.8i (0.12 g)
l-Phenylalanine1.21d 2.06i (0.34 g)
l-Proline4.78i (0.92 g)
l-Serine1.9d 3.42i (0.36 g)
l-Threonine0.42d 2.52i (0.3 g)
l-Tryptophan1d 0.5i (0.1 g)
l-Tyrosine1.1d 0.66i (0.12 g)
l-Valine4.27d 4i (0.48 g)
l-Cystine0.42d
Vitamins (μM)
 B12(cobalamin)* 7.4h 7.4 (0.01 mg)
 Riboflavin* 2.66e 0.53 (0.2 mg)
 Calcium pantothenate* 2e 0.2 (0.1 mg)
 Niacin* 14d 1.4 (0.17 mg)
 Folic acid* 0.72d 0.072 (0.032 mg)
 Pyridoxal* 2.45e 0.245 (0.05 mg)
 Pyridoxamine* 0.83
p-Aminobenzoate* 2.92d
 Biotin* 0.04e
 Thiamine* 3e
l-Ascorbic acid* (mM)2.8
 Spermine · 6H2O* 2.5
  • a Concentrations are millimolar unless otherwise indicated.

  • b *, component tested by single omission (all micronutrients and bases were omitted at once).

  • c MPLR corresponds to the rich medium composition before optimization of concentrations. Concentrations used in previously described CDMs are indicated with a footnote.

  • d According to Lauret et al. (20).

  • e According to Ledesma et al. (21).

  • f According to Sasaki and Sasaki, Abstr. Fifth Symp. Lactic Acid Bacteria.

  • g According to Jensen and Hammer (16).

  • h According to Atlan et al. (4).

  • i According to D. Roy, 1995 online communication in Lactacid Forum (http://lactacid@searn.sunet.se).