Table 5.

PrtH activity of L. helveticus CRL 1062 after growth in SCDM supplemented with different peptidesa

Addition to basal
SCDM
PrtH sp act (nmol min−1 mg of protein−1)b
None192 ± 8
1% LMMP18 ± 1
1% HMMP191 ± 8
1 mM Leu-Gly-Glyc190 ± 7
1 mM Leu-Leud189 ± 8
5 mM Leu-Leud185 ± 9
1 mM Leu-Pro191 ± 7
5 mM Leu-Pro144 ± 5
  • a Values are the means and standard deviations from three independent experiments.

  • b LDH activity was less than 0.06% the total activity in each cell extract.

  • c The PrtH activity of L. helveticusCRL 1062 after growth in SCDM with the tripeptide Val-Pro-Leu was similar.

  • d The PrtH activity of L. helveticusCRL 1062 after growth in SCDM with the dipeptides Gly-Met, Gly-Pro, Gly-Tyr, Pro-Ala, Pro-Leu, and Tyr-Gly was similar.