Table 1.

Specificity of fluorogenic PCR to detect Salmonella and E. coli O157:H7

Bacterial strainsaSerotype, species, or groupbeaegene presentcsipB-sipC genes presentcFluorogenic PCR detection
E. coli O157:H7 specificSalmonella specific
Nontoxigenic E. coli O111:H12 (2)
H21 (3)
O149:HN
O157:H43
O128:H7 (2)
H21
H47
ETECO101
O149:H10
RDECO132+
O15:HN+
EPECO55:NM (2)+
H6 (2)+
O26:NM (3)+
O111:H2 (3)+
O128:H2 (4)+
O45:H2+
O55:H7 (2)++
STECO111:NM (2)+
O111:H8+
O26:H11+
O45:H2
O113
OX3:H2
EHECO26:H11 (3)+
O111:NM+
O157:H7 (5)++
O157:NM (1)++
Salmonella Typhimurium++
Enteriditis++
Samftenberg++
Litchfield++
DT104 (2)++
B1RC8++
B1RC9++
EILC9++
E1LC10++
C1CC117++
Enterobacter E. cloacae
Hafnia H. alvie +
Klebsiella K. pneumoniae
Proteus P. vulgaris
Pseudomonas P. aeruginosa
Staphylococcus S. aureus
  • a ETEC, enterotoxigenic E. coli; RDEC, rabbit diarrheagenic E. coli; EPEC, enteropathogenic E. coli; STEC, Shiga toxin-producingE. coli; EHEC, enterohemorrhagic E. coli.

  • b Parentheses indicate the number of isolates of a serotype tested.

  • c The presence of the eae gene inE. coli and that of sipBC inSalmonella was determined by colony blot hybridization using probes specific for these genes (2, 11;http://www.bio.psu.edu/people/faculty/whittam/lab/deca/).