Table 2.

Quantification of bacteria by FISH and rRNA slot blot hybridization in Smeerenburgfjorden sediments (core A/core B)

Depth (cm)Absolute prokaryotic cell nos (109 ml−1)Total RNA, probe Uni1390 (ng of RNA ml−1)Bacteria (probe EUB338)Archaea (probe ARCH915)Eucarya (probe EUK1379)Cytophaga-Flavobacterium (probe CF319a)
% Total DAPI cell counts% Universal RNAng of RNA ml−1% Total DAPI cell counts% Universal RNAng of RNA ml−1% Universal RNAng of RNA ml−1% Total DAPI cell counts% Prokaryotic RNAng of RNA ml−1
0.252.9/2.115,658/18,42957.9/72.961.3/47.89,601/9,8456.4/3.31.7/0.9264/16919.2/26.33,010/4,8419.6/12.86.1/5.4606/481
0.753.5/3.314,750/22,86055.9/73.666.7/59.39,845/13,5641.8/1.81.3/1.2191/28212.4/15.91,829/3,62910.3/10.45.5/5.8554/799
1.253.1/3.413,943/20,44856.1/65.451.5/50.87,179/10,3912.9/1.81.0/1.0142/20625.1/30.43,500/6,2257.4/6.84.1/4.1299/433
1.754.2/4.211,449/13,03553.1/57.758.9/62.86,744/8,1832.1/1.51.2/1.1135/14223.9/15.22,734/1,9837.2/4.84.3/3.8297/315
2.254.1/3.511,276/18,64757.5/48.462.3/50.57,028/9,4171.8/1.51.2/1.0133/18818.0/20.82,026/3,8837.6/3.04.2/5.4299/522
2.753.7/3.37,795/8,31847.9/51.263.4/69.64,944/5,7911.4/0.81.2/0.993/7521.4/12.01,666/9958.5/7.14.3/3.6217/213
3.252.7/3.28,266/9,15743.7/46.656.1/69.34,638/6,3441.4/1.71.0/0.886/7325.8/12.02,129/1,2587.6/3.94.1/3.3193/215
3.753.1/3.77,896/9,34242.8/50.064.9/70.85,125/6,6131.4/1.71.1/0.687/5812.1/13.7954/1,1818.2/3.33.7/3.4192/229
4.253.5/3.45,964/6,49443.5/47.359.2/77.33,531/5,0191.4/0.41.4/0.883/5218.3/12.61,093/8954.3/2.84.9/3.1177/159
4.753.4/3.65,559/5,95228.4/42.263.4/73.53,526/4,3731.4/1.11.4/0.979/5415.3/13.8853/8114.4/1.84.2/3.2152/140
5.53.5/4.63,306/4,95626.0/36.077.7/80.62,570/3,997NDa 1.4/1.046/498.3/15.5274/7691.8/2.04.0/3.4106/137
6.53.7/4.73,345/4,89724.1/27.487.6/90.82,929/4,448ND1.6/0.955/4610.1/13.1339/6431.0/1.73.7/2.7112/122
7.54.7/3.93,283/3,97223.6/23.680.1/87.52,631/3,474ND0.9/1.231/4612.4/13.5408/5360/03.5/3.193/110
8.52.9/3.13,599/4,33128.9/22.678.5/73.92,824/3,2001.0/1.71.3/1.145/4611.7/12.9421/5590/0.53.4/3.597/112
9.53.7/3.93,199/3,58922/15.771.1/79.72,273/2,8611.0/0.91.1/1.035/3812.6/14.9402/5340/03.5/3.181/90
113.9/3.41.7/0.7
132.7/3.40.6/0.8
152.5/3.40.6/1.2
173.3/2.7
192.7/2.1
  • a ND, not determined.