Table 3.

Effects of species diversity on the formation of PCR artifactsa

No. of species in communityMean % chimerasMean error rate ± SD (%)Mean % heteroduplexes ± SDMean % total PCR artifact ± SD
4NDb 2.5 ± 1.6ND2.5 ± 1.6
7ND1.9 ± 1.91.9 ± 0.03.7 ± 1.8
10ND1.9 ± 0.03.7 ± 2.15.6 ± 2.1
  • a For each community, 54 clones were examined in triplicate.

  • b ND, not detected.