Table 1.

Transformation efficiencies inPropionibacterium strains with DNA isolated from E. coli or P. freudenreichiiATCC 6207a

Recipient strainTransformation efficiency (no. of transformants/μg of DNA)
DNA isolated from E. coliDNA isolated from P. freudenreichii
P. freudenreichii strains
 ATCC 620710–30≥108
 VTB110–30≥108
 LMG 1654510–30≥108
P. acidipropionicistrains
 DSM 1357200
 DSM 2072700
P. jensenii DSM 2053500
P. thoenii DSM 2027600
  • a Electroporation was performed at 20 kV/cm, 200 Ω, and 25 μF. The vectors used for transformation were pBRESP36A, pBRESP36B1, and pBRESP36B2.