Table 1.

Recombinant plasmids used in this work

PlasmidDescription
pCFMDerivative of pUC119 carrying a 4,896-bp SalI-KpnI fragment containingcft
pCFP Derivative of pCFM deletion from nt 1 to 1037 of the 4,896-bp SalI-KpnI fragment
pCFQDerivative of pCFM deletion from nt 408 to 1237 of the 4,896-bpSalI-KpnI fragment
pCFMΔCRE1 Derivative of pCFM deletion from nt 1 to 261 of the 4,896-bpSalI-KpnI fragment
pCFMΔCRE2 Derivative of pCFM deletion from pCFM deletion from nt 1 to 365 or 4,896-bpSalI-KpnI fragment
pCFMHis Derivative of pCFM carrying the CFT150 gene with six His at the C terminus
pECFTNDerivative of pET23a carrying the CFT107 gene with six His at the C terminus