Table 5.

Nature of mutation in most frequently detected clones

Clone no.Reference strainError position in:Base substitutionaRFLP shift
Reference strainE. coli
15C1-410331109GCGC→GCGTLostHhaI
16C1-49761049GTGC→GCGCGainedHhaI
6Tol-49941026GTGC→GCGCGainedHhaI
40Tol-412321265GCGC→TCGCLostHhaI
14B9-1210721109GCGC→GCGTLostHhaI
34B9-12540576ACGC→GCGCGained HhaI
7P39854878GCAC→GCGCGained HhaI
35P39739763GCGC→GTGCLostHhaI
  • a HhaI restriction recognition sequence, GCGC.