Table 4.

Elimination of heteroduplexes with polyacrylamide gel purification and T7 endonuclease I digestion

DNA polymeraseTreatmentMean % Chimeras ± SDMean Error rate ± SD (%)Mean % heteroduplexes ± SDMean % total PCR artifacts ± SD
Z-Taq Control8.7 ± 2.29.3 ± 0.03.7 ± 1.120.4 ± 0.0
Gel purification8.0 ± 2.77.4 ± 1.9NDa 15.4 ± 4.3
LA-Taq Control1.9 ± 1.13.1 ± 0.63.1 ± 1.28.1 ± 2.5
T7-endo Ib 5.6 ± 1.13.1 ± 0.6ND8.7 ± 0.6
  • a ND, not detected.

  • b T-endoI, T7 endonuclease I.