Table 2.

Primers used in the present study

PrimerSequence (5′ to 3′)UseSpecificity or targetReference
Bif164-fGGGTGGTAATGCCGGATGPCRBifidobacterium16S17, 19
Bif662-rCCACCGTTACACCGGGAAPCRBifidobacterium16S17, 19
Bif164-GC-fCGCCCGGGGCGCGCCCCGGGCGGGGCGGGGGCACGGGGGG-GGGTGGTAATGCCGGATGPCRBifidobacterium16SThis study
Bif662-GC-rCGCCCGCCGCGCGCGGCGGGCGGGGCGGGGGCACGGGGGG-CCACCGTTACACCGGGAAPCRBifidobacterium16SThis study
Im26-fGATTCTGGCTCAGGATGAACGPCRBifidobacterium16S15
Im3-rCGGGTGCTICCCACTTTCATGPCRBifidobacterium16S15
7-fAGAGTTTGAT(C/T)(A/C)TGGCTCAGPCREubacterial 16S18
1510-rACGG(C/T)TACCTTGTTACGACTTPCREubacterial 16S18
1401-rCGGTGTGTACAAGACCCRT-PCREubacterial 16S30
338-rCTGCTGCCTCCCGTAGGAGTSequencingEubacterial 16S18
338-fCTCCTACGGGAGGCAGCAGSequencingEubacterial 16S18
515-rATCGTATTACCGCGGCTGCTGGCACSequencingEubacterial 16S18
968-fAACGCGAAGAACCTTASequencingEubacterial 16S18
1100-rGGGTTGCGCTCGTTGSequencingEubacterial 16S18
T7TAATACGACTCACTATAGGGSequencingpGEMTPromega
Sp6GATTTAGGTGACACTATAGSequencingpGEMTPromega