Table 1.

Bacterial strains and plasmids

Strain or plasmidaOrigin or relevant characteristicsSource and/or reference
Bacterial strains
L. acidophilus ATCC 4356Type strain; human pharynxATCCb
L. acidophilus NCFMHuman intestinal isolate7
L. casei ATCC 393Type strain; cheese isolateATCC
L. delbrueckii subsp. bulgaricus 1489Cheese isolate55
L. gasseri ADHHuman intestinal isolate30 (called strain MS02)
L. gasseriATCC 33323Type strain; human isolateATCC
L. gasseri NCK 1338Human endoscopy isolate32
L. gasseri NCK 1340Human endoscopy isolate32
L. gasseri NCK 1341Human fecal isolate32
L. gasseri NCK 1342Human endoscopy isolate32
L. gasseri NCK 1343Human fecal isolate32
L. gasseri NCK 1344Human endoscopy isolate32
L. gasseriNCK 1345Human endoscopy isolate32
L. gasseri NCK 1346Human endoscopy isolate32
L. gasseri NCK 1347Human endoscopy isolate32
L. gasseri NCK 1348Human fecal isolate32
L. gasseri NCK 1349Human fecal isolate32
L. helveticus CNRZ 32Cheese isolateSteelec (8)
L. johnsonii VPI 11088ATCC 11506; origin unknownVPId
L. plantarum 299vHuman intestinal isolate1
L. reuteri DSM 20016ATCC 53609, type strain; human fecal isolateATCC
L. rhamnosusGGATCC 53103; human fecal isolateATCC
E. coli DH10BFmcrA Δ(mrr-hsdRMS-mcrBC) φ80dlacZΔM15 ΔlacX74 endA1 recA1 deoRGibco-BRLe
E. coli DH5αF φ80dlacZΔM15Δ(lacZYA-argF) U169 endA1 recA1 hsdR17(rKmK+)deoRGibco-BRL
E. coliKW1Δ(add-gus-man) hsdR hsdM+metB strA purB54
E. coli Tuner(DE3)FompT hsdSB(rBmB) gal dcm lacY1 (DE3)Novagenf
Plasmids
 pET28a(+)Kanr;E. coli expression vectorNovagen
 pTRK665pET28a(+) with cloned gusA geneThis study
 pUC18Apr; cloning vectorAmershamg
 pTRK666pUC18 with 4.5-kb L. gasseri ADH chromosomal insert; GusA+This study
 pTRK667pUC18 with 4.9-kbL. gasseri ADH chromosomal insert; GusA+This study
 pGK12ori (pWV01), Emr, Cmr; gram-positive shuttle vector31
 pTRK563Emr; Δcat-derivative of pGK12 with lacZ from pBluescript II KS(+)This study
 pTRK668pTRK563 with 4.5-kb EcoRI-PstI insert from pTRK666; GusA+This study
 pTRK664pTRK563 with T7 terminator-P6 promoter-gusAgene; GusA+This study
  • a Intermediate plasmids not shown.

  • b American Type Culture Collection.

  • c James Steele, Dept. of Food Science, University of Wisconsin—Madison.

  • d Virginia Polytechnic Institute.

  • e Gibco-BRL, Grand Island, N.Y.

  • f Novagen, Inc., Madison, Wis.

  • g Amersham Pharmacia Biotech, Piscataway, N.J.