Table 1.

16S rDNA sequences identified in anoxic water column of the Cariaco Basin in three clone libraries

Phylogenetic affiliationRepresentative clone (accession no.)No. of clones in libraryClosest relative
320 m 500 m 1,310 mTaxon Accession no. % Identity
Archaea
Crenarchaeota Car132fc (AF224859) 2Archaeon C20 U71114 98.9
 Euryarchaeota Car127c (AF224855) 1 Methanospirillum hungatei M60880 74.5
Car106b (AF224779) 1 Methanospirillum hungatei M60880 74.9
Car42fc (AF224831)a 3 Thermoplasma acidophilum M38637 74.2
Car68fc (AF224866)1 Thermoplasma acidophilum M38637 75.3
Car154fc (AF224869) 1 Thermoplasma acidophilum M38637 72.4
Bacteria
 Candidate division OP8Car60fc (AF224839) 1 OPB95 AF027060 90.3
 Candidate division OP3 Car11fa (AF224797) 1OPB2 AF027088 80.1
Car136b (AF224787)2 BD3-9 AB015551 80.9
Car30c (AF224825)1 Koll 11 AJ224540 82.6
Car40rc (AF224830) 1 Geobacter metallireducens L07834 81.2
Car119rc (AF224848) 1 BD4-9 AB015559 79.2
Car121c (AF224850) 1 Methylobacterium sp. D87985 79.1
Car124rc (AF224853) 1 Clostridrium thermocellum L09173 79.4
 Planctomycetes Car65rc (AF224864) 1 BD2-3 AB015533 85.9
Car68rc (AF224865) 1 BD2-3 AB015533 85.6
Car122fc (AF224851) 1 BD2-3 AB015533 85.2
 Fibrobacteria Car104fb (AF224872)a,b 6 SAR406 U34043 89.4
Car97fb (AF224822)b 1 SAR406 U34043 86.1
Car137rb (AF224788) 1 SAR406 U34043 90.5
Car53c (AF224775) 2 1 OCS307 U41450 87.6
Car129rc (AF224857)a 2 2Clone pG-13 AF001509 82.1
Flexibacter-Cytophaga-Bacteroides Car44rc (AF224833) 1 Bacteroides ovatus X83952 92.2
Car56rb (AF224809) 1 1 Sphingobacterium heparinum M11657 98.5
Car82fb (AF224811) 1 Flavobacterium balustinum D14016 92.1
Car114fc (AF224844)1 Flavobacterium balustinum D14016 99.2
Car116rc (AF224845) 1 Acinetobacter sp. Y15852 73
Verrucomicrobia Car98rb (AF224823) 1Clone WCHB1-41 AF050560 86.9
 Proteobacteria
  Nonaffiliated proteobacteria Car731c (AF224870) 2 A51p X91474 88.2
Car63a (AF224801) 1 Clone MUG16 AB011308 73.2
Car33fb (AF224808) 2 Lucina floridana symbiont L25707 86.8
  β-Proteobacteria Car117fc (AF224846) 1 Alcaligenes faecalis AJ277669 97.5
  γ-Proteobacteria Car16fa (AF224800) 1 Thiomicrospira sp. L40811 86.7
Car36rc (AF224827)a 2 Acyrthosiphon pisum symbiont AB033779 93.0
Car128fc (AF224856)c–e 4 1Clone UN106 D63592 99.4
Car91fb (AF224817)c 1 Clone UN106 D63592 99.7
Car141fb (AF224791)d 1 Clone UN106 D63592 99.3
Car149fc (AF224862)e 1 Clone UN106 D63592 99.7
Car39fc (AF224829) 1 Citrobacter farmeri AF025371 93.4
Car70c (AF224867)a 3 11 PVB18 U15114 99.1
Car43fc (AF224832) 1 PVB18 U15114 95.6
Car140fb (AF224790) 2 PVB18 U15114 95.5
Car92rb (AF224818) 1 1PVB18 U15114 96.5
Car123rc (AF224852) 1 Photobacterium phosphoreum D25310 95.9
  δ-Proteobacteria Car45fc (AF224834) 1 2Clone SB-30 AF029048 96.2
Car102rb (AF224777)1 Clone SB-30 AF029048 95.4
Car148rc (AF224861) 1 Clone SB-30 AF029048 88.4
Car113fc (AF224843) 1 Clone SB-30 AF029048 94.9
Car99rb (AF224824) 1 Clone SB-30 AF029048 91.4
Car81fb (AF224810) 1Clone A48u X91471 90.0
Car86rb (AF224813)1 Clone NKB19 AB013271 81.3
Car93fb (AF224819) 1 Clone BD7-15 AB015588 93.4
Car95fb (AF224821) 1 Clone SB-21 AF029045 93.9
Car7ra (AF224796) 1 SAR276 U65915 88.9
Car150fc (AF224868) 1 RFLP25 AF058007 95.7
Car103rb (AF224871) 2 Bdellovibrio stolpii AJ288899 90.5
Car147fc (AF224860) 1 Strain S16 Z69320 92.8
Car37fc (AF224828) 2 Desulfobacter postgatei M26633 83.6
  ɛ-Proteobacteria Car153a (AF224805)a 51 Clone NKB12 AB013264 94.6
CarB13fa (AF224804) 1Clone NKB12 AB013264 96.7
Car29rb (AF224806)3 Clone NKB12 AB013264 95.8
Car55fb (AF224807) 6 Clone NKB12 AB013264 95.6
Car59rc (AF224838) 4Clone NKB12 AB013264 95.4
Car125fc (AF224854) 1 Clone NKB12 AB013264 95.6
Car13a (AF224799) 1 Clone BD7-6 AB013264 94.2
Car2a (AF224795) 1Thiomicrospira denitrificans L40808 94.2
Car107fb (AF224780) 1Thiomicrospira denitrificans L40808 91.7
Car12fa (AF224798) 2 Arcobacter nitrofigilis L14627 93.9
Chimeras 1 56
Total 61 56 65
  • a Representative of a group of closely related sequences (>99% identity).

  • b Car104fb and Car97fb exhibit 98.7% identity.

  • c Car128fc and Car91fb exhibit 98.2% identity.

  • d Car128fc and Car141fb exhibit 98.8% identity.

  • e Car128fc and Car149fc exhibit 98.3% identity.