Table 3.

Major archaeal lineages represented in 16S rDNA librariesa

Archaea Presence in or absence from indicated sample (% of total organisms) in 16S rDNA library:
Eel-TAbEel-BAbEel-36a SB-24a SB-17a SB-7a pISA (39)GBcMT/C (29) APA (46)JTB (26)
Eel River BasinSanta Barbara Basin
Eel-Hpc4 (0–4 cm)Eel-Hpc4d (20–22 cm)Eel-pc36d (4–7 cm)SB-pc24d (13–16 cm)SB-pc17 (4–7 cm)SB-pc7 (4–7 cm)Hydrothermal vent sediments from JapanSeep sediments from
Guaymas Basin
Coastal salt marsh, Essex,
United Kingdom
Deep-sea sediments from the AtlanticCold seep sediments from the Japan Trench
ANME-1 + (55) + (84)+ (7.5) + (18) ++
ANME-2 + + (16) + (71) + (67) + (22) + +
Methanosarcinales + (6) + (9) +
Crenarchaeota + (2) + (2) + (4) + + +
Thermoplasmales + + (2) + (3) + (41) + + + ++
  • a +, sequence type detected; −, not detected. Numbers in parentheses beside 16S rDNA library designations (pISA, GB, MT/C, APA, and JTB) are reference numbers.

  • b Data were previously reported in reference18.

  • c Data are from Andreas Teske (personal communication).

  • d δ-13C-depleted archaeal lipid biomarkers detected in methane seep sediment samples included archaeol,sn-2-hydroxyarchaeol, dihydrophytol, unsaturated PMI, and crocetane. Archaeal biomarkers were not detected in sample SB-17a or in nonseep control sample SB-7a. Crocetane was not detected in sample Eel-BA. δ-13C values of these lipids ranged from −81 to −129‰.