Table 1.

Characterization of 57 E. coli O157:H7 isolates from fresh fecal pats collected from southwest Kansas beef cattle feedlotsa

CollectionFeedlotPen IDPen typePCR characterizationLaneRAPD patternFeedlot strain no.
eaeAhlyAstx1stx2TP
3M128Home321214
7M40Home++++3111442
13M114Home2915125
13M1022Hospital30b141313
1R113Home++++4522
1R300Home+++4411434
1R505cHome+++49127
1R505cHome+++4821620
1R47Hospital+++43228
1R47cHospital471222
1R47cHospital++++46111537
8R402Home44
8R81cHome4113216
8R81cHome4212248
11R45cHospital++++3921024
11R45cHospital401226
12R256Hospital382211
15R113Home362346
16R97Buller+++341238
16R107Home++++3314229
16R43Hospital3510310
1S409Home+++271239
2S223Home+++2611018
5S303Home+++231221
8S301Home15184
8S228cHome++++2011123
8S228cHome+++211227
8S228cHome++++221333
8S228cHome+++1811054
8S228cHome+++19b110
8S409cHome (W)++++17391
8S409cHome (W)164243
13S110Home14572
13S15Hospital12b6532
13S19Hospital13b1615
14S18Hospital11b7547
S20Buller28b16251
2V306Buller++++62325
2V53Home+++72252
2V75Home81336
2V61cHome+++91428
2V61cHome102230
4V306cBuller++++242109
4V306cBuller+++252235
4V533Home57217
7V83Home+++4b2319
10V306Buller49
10V16Home38240
10V71Home53
10V24cHome1913
10V24cHome+21212
12V43Home+++541231
13V433Home532245
14V52Home522250
15V29Home37236
15V52Home512241
16V43Home50b102
  • a Isolates were collected from pen floors at four southwest Kansas beef cattle feedlots (V, S, M, and R) every 3 weeks for 11 months, resulting in 16 collections. Data are sorted by feedlot and collection within feedlot. All isolates were from fecal pat samples, except for two from a water (W) sample. Lane numbers correspond to lanes in Fig. 1 in left-to-right, top-to-bottom order. RAPD T is the pattern identified from total DNA; RAPD P is the pattern identified from plasmids of the isolate. The genes amplified by PCR were eaeA (intimin), hlyA (hemolysin),stx1 (Shiga toxin I), and stx2 (Shiga toxin II). + and −, presence or absence, respectively, of the gene. Three isolates were not characterized by RAPD. Blank spaces indicate missing data. Feedlot strain number can be used to cross-reference additional information about these isolates (19).

  • b Strain was resistant to five or more antibiotics.

  • c Multiple isolates from the same sample.