Table 1.

Summary of samples used in the construction of SSU rDNA libraries and their physical and chemical descriptions

SampleaDepth (cm)Latitude, longitude (°) Physical or chemical description SO4 (mM) H2S (mM)rDNA library designation PCR primers No. of clones screened
Eel-Hpc4 0–440.47, −124.35Bacterial matNAe NA Eel-TEB27f-U1492r 52
Eel-Hpc4 20–22 40.47, −124.35 Bacterial matNA NAEel-BE B27f-U1492r 121
Eel-pc36 4–7 40.48, −124.36 Clam patch, high CH4 20 +d Eel-36eB27f-U1492r 18
Eel-pc36 4–7 40.48, −124.36Clam patch, high CH4 20 + Eel-36aA20f-U1492r 121
SB-pc24 14–16 34.10, −119.99Calyptogena clam patch 5.9b 11c SB-24e B27f-U1492r 91
SB-pc24 14–16 34.10, −119.99 Calyptogena clam patch5.9 11 SB-24a A20f-U1492r 90
SB-pc176–8 34.25, −119.97 Bacterial mat 11.5 10SB-17a A20f-U1492r 77
SB-pc7 12–14 34.27, −119.96 Reference core, background 28  0 SB-7aA20f-U1492r 25
  • a SB, Santa Barbara Basin; Eel, Eel River Basin. Water depth ranged between 519 and 556 m for both the Santa Barbara and Eel River basin sites.

  • b SO4 2− values for Santa Barbara Basin samples were provided by Jon Martin (personal communication).

  • c H2S values for Santa Barbara Basin samples were provided by Jim Barry (personal communication).

  • d +, H2S detected but not quantified.

  • e NA, not available.