Table 5.

Major bacterial lineages represented in 16S rRNA gene libraries from methane seeps

OrganismsClosest relative Presence in or absence from indicated sample (% of total organisms) in 16S rDNA librarya:
SB-24eEel-BE Eel-TEcEel-36ecJTB (26)GCAbTAY-NAYA (6) sva (47)
Santa Barbara Basin SB-pc24 (13–16 cm)Eel River Basin Eel-Hpc4 (20–22 cm)Eel River Basin
Eel-pc36 (4–7 cm)
Santa Barbara Basin
SB-pc24 (13–16 cm)
Cold seep sediment
from Japan
Oil seep sedimentsAntarctic fjord sediment from Taynaya BayShelf sediments from the Arctic Ocean
δ-Proteobacteria + (25)+ (36.4) + + ++ + +
ɛ-ProteobacteriaS-oxidizing symbionts + (26) + + +
γ-Proteobacteria (S-oxidizing symbionts + others) + (4.3) + (3.3) + + + ++ +
α-Proteobacteria Rhizobium spp. + + + +
Planctomyces Pirellula spp. + (3.3) + (1) + +
FBCd Cytophaga spp. + (3.3) + (6.6) + + + +
Graciliutes Haloanaerobiumspp. + (2.5) +
Gram-positive bacteria Propionigenium spp. + (4.1) + + + + +
Green nonsulfur organisms + (5.5) + +
Holophaga Acidobacterium spp. + (3.3)+ (2.5) + + + +
Candidate division OP-9 + (3.3) + (21) ++
Candidate division OP-11 + (4.1)
Spirochetes+ (5.5)
  • a Numbers in parentheses beside library designations are reference numbers.

  • b Hydrocarbon seep. Data are from K. R. O'Neill (GenBank database accession no. AF154080 to AF154106).

  • c Based on a partial library screening of Eel-TE (52 clones) and Eel-36e (18 clones).

  • d FBC,Flexibacter-Bacteroides-Cytophaga division.