Table 1.

A selection of GFP expression vectors for transformation of filamentous fungi

Construct (reference)gfp genePromoter driving gfp, originExpression, localization featuresFungal species transformed
pOTEF-SG (54) SGFP Otef,modified tef, U. maydis Constitutive, no localization U. maydis
pMFA1-SG (54) SGFP mfa1, U. maydis Mfa1 phermone-inducible, no localization U. maydis
pCRGFP2 (6) SGFP crg1, U. maydis l-Arabinose-inducible, d-glucose andd-xylose repressible, no localization U. maydis
pNUC'EM2 (20) EGFP cmv, human cytomegalovirusConstitutive, SV40 nuclear localization signal A. flavus
pCT74 (this review) SGFP ToxA, P. tritici-repentis Constitutive, no localization C. magna, P. tritici-repentis, S. sclerotiorum, Colletotrichum trifolii, V. dahliae, A. alternata, B. cinerea, C. sativus, F. sambucinum
pCMB32 (22) SGFP alcA, A. nidulans Alcohol-inducible, no localization A. nidulans
pCMB41 (22) SGFP alcA, A. nidulans Alcohol-inducible, plant chitinase (mgfp5) signal peptide, ER retention signal (His-Asp-Glu-Leu) A. nidulans
pCMB42 (22) SGFP alcA, A. nidulans Alcohol-inducible, GAL4BD fusion for nuclear localization A. nidulans
pRS31 (57) SGFP gpd, A. niger Constitutive, StuA fusion for nuclear localization A. nidulans
pRS54 (56) SGFP gpd, A. niger Constitutive, citrate synthase fusion for mitochondrial localization A. nidulans
gGFP (38, 44) SFGP gpd, A. nidulans Constitutive, no localization C. heterostrophus, A. nidulans
tGFP (38) SGFP trpC, A. nidulans Constitutive, no localization C. heterostrophus
pTEFEGFP (2, 61) EGFP tef, A. pullulans Constitutive, no localization A. pullulans, T. harzianum
pVW2 (62) EGFP ham 34, Bremia lactucae Constitutive, no localization Phytophthora palmivora
pAHB6 (6) SGFP hsp70, B. lactucae Basal level constitutive, heat inducible, no localization Phytophthora parasitica var. nicotianae