Table 1.

Corynebacterium ORFs printed onto microarrays and significant changes in gene expression during lysine production

GeneAssigned function of gene productPCR product length (bp)Accession no. or sourceFold inductiona
aat aspartate aminotransferase1,050 E16763 ns
accBC acyl-CoA carboxylase (biotinylated subunit)1,050 U35023 ns
accD acetyl-CoA carboxylase (transcarboxylase subunit)1,100L. B. Willis, unpublished data ns
aceA isocitrate lyase1,040 L28760 ns
aceB malate synthase1,090 L27123 ns
amt ammonium transport system1,340 X93513 ns
amtR repressor of (methyl) ammonium uptake system590 AJ133719 ns
argS arginyl-tRNA synthase1,100 E16355 ns
asd aspartate-semialdehyde dehydrogenase900 L16848 ns
ask aspartokinase1,060 L16848 ns
bioB biotin synthase890 U31281 ns
dapA dihydrodipicolinate synthase850 Z21502 ns
dapB dihydrodipicolinate reductase700 Z21502 ns
dapD tetrahydrodipicolinate succinylase540 AJ004934 ns
dapE succinyl-diaminopimelate desuccinylase1,050 X81379 ns
ddc diaminopimelic acid decarboxylase1,070 E16355 ns
ddh Embedded Image eso-diaminopimelated-dehydrogenase950 Y00151 ns
dtsR1 acyl-CoA carboxylase (transcarboxylase subunit)1,080 AB018531 +2.8
dtsR2 acyl-CoA carboxylase (transcarboxylase subunit)1,080 AB018531 ns
ectP transport of ectoine, glycine betaine, and proline1,090 AJ001436 ns
fda fructose-bisphosphate aldolase1,000 X17313 ns
gap glyceraldehyde-3-phosphate dehydrogenase970 X59403 ns
gdh glutamate dehydrogenase1,020 X59404 ns
glnA glutamine synthetase1,130 AF005635 +1.8
gltA citrate synthase1,050 X66112 −2.6
gltB glutamine 2-oxoglutarate aminotransferase (large subunit)1,080 AB024708 ns
glyA serine hydroxymethyltransferase1,040 E12594 ns
gpd 6-phosphogluconate dehydrogenase990 E13660 ns
hom homoserine dehydrogenase1,040 Y00546 ns
icd monomeric isocitrate dehydrogenase1,060 X71489 ns
ilvA threonine dehydratase1,030 L01508 ns
ilvB AHAS (large subunit)1,020 L09232 +1.8
ilvC acetohydroxy acid isomeroreductase930 L09232 ns
ilvN AHAS (small subunit)470 L09232 ns
leuB 3-isopropylmalate dehydrogenase990 Y09578 ns
lysE lysine export670 X96471 +1.8
lysG lysine export regulator810 X96471 ns
metA homoserineO-acetyltransferase1,090 AF052652 ns
ndh NADH dehydrogenase1,050 AJ238250 ns
odhA 2-oxoglutarate dehydrogenase1,250 1,050b D84102 −3.7, −3.2 b
panC pantothenate β-alanine ligase780 X96580 ns
pgk phosphoglycerate kinase1,060 X59403 ns
ppc phosphoenolpyruvate carboxylase1,040 E16358 ns
proP proline/ectoine uptake1,050 Y12537 ns
pta phosphate acetyltransferase930 X89084 −2.2
ptsM phosphoenolpyruvate sugar phosphotransferase1,060 L18874 ns
pyc pyruvate carboxylase1,190 AF038548 ns
pyk pyruvate kinase1,060 L27126 +1.5
thrB homoserine kinase870 Y00546 ns
thrC threonine synthase1,140 X56037 ns
tpi triosephosphate isomerase750 X59403 ns
zwf glucose-6-phosphate dehydrogenase1,040 E13655 ns
  • a Relative to the mean and based upon analysis of the AR data set for each gene; ns, no significant change in expression.

  • b Two different PCR products were prepared fromodhA and printed separately on the array.