Table 1.

E. coli strains transformed with pDEFGa

E. colistrainVectorKanamycin resistanceColony phenotypeSequence of csgD promoter −10 on pDEFG
Top 10Red
pCR2.1-TOPO+Red
pDEFG+Red5′ TAGAAT
43895OWpCR2.1-TOPO+White
pDEFG+RedbTAGATT
CuredcWhite
43895FWpCR2.1-TOPO+White
pDEFG+RedbTAGATT
CuredWhite
43894OWpCR2.1-TOPO+White
pDEFG+RedbTAGATT
CuredWhite
43894FWpCR2.1-TOPO+White
pDEFG+RedbTAGATT
CuredWhite
84-2pCR2.1-TOPO+White
pDEFG+RedbTAGATT
CuredWhite
161-2pCR2.1-TOPO+White
pDEFG+RedbTAGATT
CuredWhite
Tarr1ApCR2.1-TOPO+White
pDEFG+RedbTAGATT
CuredWhite
Tarr4ApCR2.1-TOPO+White
pDEFG+RedbTAGATT
CuredWhite
  • a pDEFG is pCR2.1-TOPO (kmr) plus the recombinant csgDEFGoperon of 43894OR1.

  • b Original transformed plates contained 90% red and 10% white colonies.

  • c Strains were cured of pDEFG by propagation on nonselective media.