Table 4.

Quantification of rumen bacteria by real-time PCR during diet shift

Bacteria targetedDNA concna
Day 0Day 3Day 28
P. ruminicola 29.45 ± 3.6205.2 ± 38.410.94 ± 1.9
P. bryantii 0.284 ± 0.174.77 ± 14.92.865 ± 0.5
F. succinogenes 12.32 ± 0.80.597 ± 0.00.215 ± 0.0
S. ruminantium- M. multiacida 7.114 ± 1.360.40 ± 6.616.92 ± 2.7
S. bovis 0.232 ± 0.115.63 ± 2.80.104 ± 0.0
T. bryantii 0.072 ± 0.00.043 ± 0.00.011 ± 0.0
E. ruminantium 0.585 ± 0.10.042 ± 0.00.048 ± 0.0
A. lipolytica 0.137 ± 0.10.440 ± 0.20.095 ± 0.0
S. dextrinosolvens 0.468 ± 0.10.733 ± 0.1NDb
R. flavefaciens 2.891 ± 0.70.260 ± 0.00.324 ± 0.1
  • a Day 0, before experiment, animals maintained on basal hay diet; day 3, animals fed high-grain diet for 3 days; day 28, animals fed high-grain diet for 28 days. The DNA concentration was measured as millimoles of 16S rDNA per milligram of total rumen DNA ± standard deviation (n = 4) .

  • b ND, not detected.