Table 1.

Summary of data on Nitrosospira sp. strain NPAV from this studya

SubstrateInoculumInitial pHFinal concn of ammonium (μg of N ml−1)Final concn of nitrite (monoculture) or nitrite + nitrate (coculture) (μg of N ml−1)Specific rate of product formation (h−1)Final pH
AmmoniaMonoculture6.0No growthNo growthNANA
AmmoniaMonoculture7.022.7 (0.27)5.31 A (0.36)0.0012 A (0.0004)5.17 (0.07)
AmmoniaMonoculture7.516.5 (1.80)5.35 A (2.03)0.0028 A (0.0010)6.57 (0.48)
AmmoniaCoculture6.0No growthNo growthNANA
AmmoniaCoculture7.021.8 (0.28)2.01 (0.24)0.0068 A (0.0023)4.37 A (0.27)
AmmoniaCoculture7.511.1 (1.01)7.48 (0.96)0.0104 A (0.0013)4.37 A (0.22)
UreaMonoculture4.016.4 A (0.13)13.5 A (0.16)0.021 A (0.00029)5.43 AB (0.03)
UreaMonoculture5.015.8 AB (0.66)15.0 B (0.59)0.022 A (0.00023)5.57 CD (0.03)
UreaMonoculture6.014.5 B (0.38)13.4 A (0.47)0.022 A (0.00015)5.50 ACF (0.06)
UreaMonoculture7.09.38 C (0.70)16.1 B (1.01)0.033 (0.00035)5.73 E (0.03)
UreaMonoculture7.59.60 C (0.36)21.2 (0.16)0.014 (0.00811)5.67 BDEF (0.07)
UreaCoculture6.016.1 A (0.3)14.4 (0.53)0.030 A (0.0054)4.47 (0.03)
UreaCoculture7.011.8 AB (2.6)16.2 (2.71)0.009 (0.0018)5.90 A (0.65)
UreaCoculture7.510.2 B (0.2)19.1 (0.15)0.027 A (0.0064)5.00 A (0.10)
Urea (MES buffer)Coculture6.21.18 (0.17)28.2 (0.35)0.0172 A (0.0005)6.2
Ammonia + urea (MES buffer)Coculture6.222.3 (0.89)33.9 (0.32)0.0195 A (0.0008)6.2
  • a Final product concentrations, specific product formation rates, and final pH values during growth ofNitrosopira sp. strain NPAV in monoculture or in coculture with an acidophilic nitrite oxidizer, Nitrobacter sp. strain NHB1, during batch growth in buffered or unbuffered media at a range of initial pH values. Values with identical letters beside them did not vary significant (P > 0.05) with pH within a particular treatment. Values in parentheses are standard errors.

  • b NA, not available.