Table 2.

Times and temperatures applied for the RTQ-PCR configuration using the TaqMan probe and the LightCycler

ParameterTemp (°C)Time(s) (s)Slope (°C/s)Acquisition mode
Denaturation95120/600a 20None
Amplification (45 cycles)95220None
626010Single
Cooling40520None
  • a Hot-start PCR.