Table 2.

Transformation of G. sulfurreducens with a variety of plasmids

PlasmidReplicon(s)Host specificityAntibiotic resistanceNo. of transformantsa per μg of DNAStability (half-life in absence of antibiotic selection)
pJRD215IncQBroadKanrStrr (1.98 ± 0.32) × 105 (11)∼60 generations
(2.96 ± 1.08) × 107 (3)c
pJRC2IncQBroadChlrStrr (4.06 ± 2.31) × 103 (3)Not determined
pCD342IncQBroadKanr (1.24 ± 0.37) × 104 (3)Not determined
pBBR1MCS-2NDb BroadKanr (2.79 ± 0.41) × 104 (3)<10 generations
pBMK7pMB1, pBG1 E. coli, Desulfovibriospp.Kanr 0 (5)Not determined
  • a Data are means ± standard errors, and the number of experiments performed is indicated in parentheses. The number of transformants per microgram of DNA was determined by counting kanamycin-resistant colonies except in the case of pJRC2, in which chloramphenicol-resistant colonies were counted. Unless otherwise indicated, G. sulfurreducens was transformed with plasmid DNA purified from E. coli strain JM109 or DH5α. The stability of pJRD215 and pBBR1MCS-2 was determined as described in Materials and Methods.

  • b ND, the incompatibility group of pBBR1MCS-2 has not been defined (2).

  • c The number of transformants obtained when pJRD215 was purified from G. sulfurreducens.