Table 1.

Bacterial strains and plasmids

Strain or plasmidRelevant characteristicsaSource or reference
Strain
P. putidaPaW340CreDmp C2,3O TrpStrr 9
P. putidaPaW340::touR Tehr P. putida PaW340 derivative; contains a copy of touRintegrated in the chromosomeThis work
E. coliJM109 recA1 hsdR17 thi Δ(lac-proAB) (F′ traD36 proAB lacI q ZΔM15)30
Plasmid
 pGEM-3ZApr; cloning vectorPromega Corp.
 pGEM-7ZApr; cloning vector, compatible with pLAFR vectorsPromega Corp.
 pJB3Km1Kmr; cloning vector5
 pUC19Apr; cloning vector24
 pFB3401Tcr; 28-kb EcoRI fragment fromP. stutzeri OX1 chromosome cloned in pLAFR13
 pFB3411Tcr; 22.4-kb EcoRI fragment from P. stutzeri OX1 chromosome cloned in pLAFR13
 pFB1021Tcr; partial deletion of pFB34113
 pFB1036Tcr; partial deletion of pFB34113
 pRZ7085Apr; pFB3401 8.5-kb ApaI fragment cloned in pGEM-7ZThis work
 pJEX159Kmr; pFB3401 5.9-kbEcoRI-XbaI fragment cloned in pJB3Km1This work
 pJSX148Kmr; pJEX159 4.8-kbSacI-XbaI fragment cloned in pJB3Km1This work
 pBZ3120Apr; pFB3401 2-kbBamHI-XhoI fragment cloned in pGEM-3ZThis work
 pVS1934Apr; pFB3411 3.4-kbXbaI-SacI fragment cloned in pUC19This work
 pVS1921Apr; pFB3411 2.1-kbMluI-SmaI fragment cloned in pUC19This work
  • a Abbreviations: Cre, o-, m-, p-cresol; Dmp, 2,3-dimethylphenol and 3,4-dimethylphenol; Trp, tryptophan; Str, streptomycin; Tc, tetracycline; Km, kanamycin; Ap, ampicillin; Teh, tellurite.