Table 1.

Oligonucleotide sequences used in this study

PrimerSequence (5′ to 3′)Saccharomyces cerevisiae positionsSpecificityReference
Set Aa
 Euk1ACTGGTTGATCCTGCCAG4 to 20Eukarya48
 Euk516r-GCb ACCAGACTTGCCCTCC563 to 548Eukarya2
Set B
 Euk1209f-GCc CAGGTCTGTGATGCCC1423 to 1438Eukarya14
 Uni1392rACGGGCGGTGTGTRC1641 to 1627Universal22
Set C
 EukAAACCTGGTTGATCCTGCCAGT1 to 21Eukarya33
 EukBTGATCCTTCTGCAGGTTCACCTAC1795 to 1772Eukarya33
  • a Set A was also used for T-RFLP analysis, using hexachlorofluorescein-labeled Euk1A and Euk516r without the GC clamp.

  • b The GC clamp sequence is CGCCCGGGGCGCGCCCCGGGCGGGGCGGGGGCACGGGGGG.

  • c The GC clamp sequence is CGCGCGCCGCGCCCCGCGCCCGTCCCGCCGCCCCCGCCCG.