Table 1.

Times and temperatures applied for the RTQ-PCR configuration using SYBR Green I and the LightCycler

ParameterTemp(s) (°C)Time(s) (s)Slope (°C/s)Acquisition mode
Denaturation95120/600a 20None
Amplification (45 cycles)95220None
60/62520None
721220Single
Melting95020None
681520None
9500.2Step
Cooling40520None
  • a Hot-start PCR.