Table 3.

Effect of growth temperature on QSM production

Growth temp (°C)aCell density at 24 h (CFU/ml)Morphology results (mean % ± SD)bobtained with used supernatant
23(2.4 ± 0.2) × 10855 ± 6 (Y)
44 ± 5 (M)
27(3.2 ± 0.4) × 10871 ± 3 (Y)
28 ± 2 (M)
30(3.7 ± 0.2) × 10875 ± 2 (Y)
23 ± 2 (M)
33(2.8 ± 0.1) × 10884 ± 3 (Y)
16 ± 3 (M)
37(3.6 ± 0.1) × 10899 ± 2 (Y)
1 ± 2 (M)
40(4.0 ± 0.1) × 10794 ± 2 (Y)
6 ± 2 (M)
43(4.0 ± 0.8) × 10772 ± 3 (Y)
27 ± 3 (M)
  • a Cultures were inoculated to a cell density of 107 per ml in GPP and incubated at the indicated temperature for 24 h on a New Brunswick Scientific G52 shaker at 125 rpm.

  • b Growth morphology-based bioassay was done as described in Fig. 1 (all at 37°C) using QSM from spent media produced by growth on GPP at the indicated temperatures. “Y” and “M” are as defined in Table 1, footnote a.